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63× oil objective zeiss lsm980 airyscan 2 microscope with environmental chamber  (Carl Zeiss)
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Carl Zeiss 63× oil objective zeiss lsm980 airyscan 2 microscope with environmental chamber
63× Oil Objective Zeiss Lsm980 Airyscan 2 Microscope With Environmental Chamber, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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environmental chamber microscope  (Carl Zeiss)
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Carl Zeiss environmental chamber microscope
Environmental Chamber Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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environmental chamber microscope - by Bioz Stars, 2026-03
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microscope-mounted environmental chamber  (Okolab USA Inc)
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Okolab USA Inc microscope-mounted environmental chamber
Microscope Mounted Environmental Chamber, supplied by Okolab USA Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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eclipse ti-e inverted widefield microscope full environmental chamber  (Nikon)
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Nikon eclipse ti-e inverted widefield microscope full environmental chamber
(A) Live-cell imaging using Mitotracker DeepRed FM and a 100x objective of Nikon-Eclipse <t>Ti-E</t> inverted wide-field <t>microscope</t> equipped with an environmental chamber. Bottom panels, higher magnification. Left-bottom panel, quantification of mitochondrial branch length in µM from 90-100 control and USP14 deleted cells. Scale bar: 20µM. Typical experiment is shown and was repeated three times with similar results. (B) EM imaging was done as described in Methods. Note elongated mitochondria (MT) in USP14 deleted cells. Red stars * mark the presence of electron-dense lysosomes/ autophagosomes/ vesicles that were increased in the USP14-deleted cells compared with controls. N represents nuclear compartment. The experiment was repeated with similar results. (C) Left panel, immunoblot using an OXPHOS antibody cocktail. Right panel, quantification of the densitometry ratio of CI-CV subunits (CI-NDUFB8, CII-SDHB, CIII-UQCRC2, CIV-MTCO1 and CV-ATP5A) normalized to β-actin. Values are means ± S.E.M. *p ≤ 0.05, ns= not significant. n=3. (D) Live-cell imaging using CellROX Green and a 20x objective of EVOS FL microscope to visualize reactive oxygen species (ROS). Note increases of ROS in USP14-deleted cells Control cells treated with 5µM H 2 O 2 for 90 min served as a positive control for oxidative stress. Top, CellROX Green. Bottom, phase-contrast images. Scale bar: 200µM. Typical experiment is shown and was repeated three times with similar results.
Eclipse Ti E Inverted Widefield Microscope Full Environmental Chamber, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eclipse ti-e inverted widefield microscope full environmental chamber/product/Nikon
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eclipse ti-e inverted widefield microscope full environmental chamber - by Bioz Stars, 2026-03
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microscope-associated environmental chambers  (Okolab USA Inc)
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Okolab USA Inc microscope-associated environmental chambers
(A) Live-cell imaging using Mitotracker DeepRed FM and a 100x objective of Nikon-Eclipse <t>Ti-E</t> inverted wide-field <t>microscope</t> equipped with an environmental chamber. Bottom panels, higher magnification. Left-bottom panel, quantification of mitochondrial branch length in µM from 90-100 control and USP14 deleted cells. Scale bar: 20µM. Typical experiment is shown and was repeated three times with similar results. (B) EM imaging was done as described in Methods. Note elongated mitochondria (MT) in USP14 deleted cells. Red stars * mark the presence of electron-dense lysosomes/ autophagosomes/ vesicles that were increased in the USP14-deleted cells compared with controls. N represents nuclear compartment. The experiment was repeated with similar results. (C) Left panel, immunoblot using an OXPHOS antibody cocktail. Right panel, quantification of the densitometry ratio of CI-CV subunits (CI-NDUFB8, CII-SDHB, CIII-UQCRC2, CIV-MTCO1 and CV-ATP5A) normalized to β-actin. Values are means ± S.E.M. *p ≤ 0.05, ns= not significant. n=3. (D) Live-cell imaging using CellROX Green and a 20x objective of EVOS FL microscope to visualize reactive oxygen species (ROS). Note increases of ROS in USP14-deleted cells Control cells treated with 5µM H 2 O 2 for 90 min served as a positive control for oxidative stress. Top, CellROX Green. Bottom, phase-contrast images. Scale bar: 200µM. Typical experiment is shown and was repeated three times with similar results.
Microscope Associated Environmental Chambers, supplied by Okolab USA Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microscope-associated environmental chambers/product/Okolab USA Inc
Average 90 stars, based on 1 article reviews
microscope-associated environmental chambers - by Bioz Stars, 2026-03
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ti-e microscope fitted with the perfect focus system and an environmental chamber  (Nikon)
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Nikon ti-e microscope fitted with the perfect focus system and an environmental chamber
(A) Live-cell imaging using Mitotracker DeepRed FM and a 100x objective of Nikon-Eclipse <t>Ti-E</t> inverted wide-field <t>microscope</t> equipped with an environmental chamber. Bottom panels, higher magnification. Left-bottom panel, quantification of mitochondrial branch length in µM from 90-100 control and USP14 deleted cells. Scale bar: 20µM. Typical experiment is shown and was repeated three times with similar results. (B) EM imaging was done as described in Methods. Note elongated mitochondria (MT) in USP14 deleted cells. Red stars * mark the presence of electron-dense lysosomes/ autophagosomes/ vesicles that were increased in the USP14-deleted cells compared with controls. N represents nuclear compartment. The experiment was repeated with similar results. (C) Left panel, immunoblot using an OXPHOS antibody cocktail. Right panel, quantification of the densitometry ratio of CI-CV subunits (CI-NDUFB8, CII-SDHB, CIII-UQCRC2, CIV-MTCO1 and CV-ATP5A) normalized to β-actin. Values are means ± S.E.M. *p ≤ 0.05, ns= not significant. n=3. (D) Live-cell imaging using CellROX Green and a 20x objective of EVOS FL microscope to visualize reactive oxygen species (ROS). Note increases of ROS in USP14-deleted cells Control cells treated with 5µM H 2 O 2 for 90 min served as a positive control for oxidative stress. Top, CellROX Green. Bottom, phase-contrast images. Scale bar: 200µM. Typical experiment is shown and was repeated three times with similar results.
Ti E Microscope Fitted With The Perfect Focus System And An Environmental Chamber, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ti-e microscope fitted with the perfect focus system and an environmental chamber/product/Nikon
Average 90 stars, based on 1 article reviews
ti-e microscope fitted with the perfect focus system and an environmental chamber - by Bioz Stars, 2026-03
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microscopes featuring environmental chambers  (Carl Zeiss)
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Carl Zeiss microscopes featuring environmental chambers
(A) Live-cell imaging using Mitotracker DeepRed FM and a 100x objective of Nikon-Eclipse <t>Ti-E</t> inverted wide-field <t>microscope</t> equipped with an environmental chamber. Bottom panels, higher magnification. Left-bottom panel, quantification of mitochondrial branch length in µM from 90-100 control and USP14 deleted cells. Scale bar: 20µM. Typical experiment is shown and was repeated three times with similar results. (B) EM imaging was done as described in Methods. Note elongated mitochondria (MT) in USP14 deleted cells. Red stars * mark the presence of electron-dense lysosomes/ autophagosomes/ vesicles that were increased in the USP14-deleted cells compared with controls. N represents nuclear compartment. The experiment was repeated with similar results. (C) Left panel, immunoblot using an OXPHOS antibody cocktail. Right panel, quantification of the densitometry ratio of CI-CV subunits (CI-NDUFB8, CII-SDHB, CIII-UQCRC2, CIV-MTCO1 and CV-ATP5A) normalized to β-actin. Values are means ± S.E.M. *p ≤ 0.05, ns= not significant. n=3. (D) Live-cell imaging using CellROX Green and a 20x objective of EVOS FL microscope to visualize reactive oxygen species (ROS). Note increases of ROS in USP14-deleted cells Control cells treated with 5µM H 2 O 2 for 90 min served as a positive control for oxidative stress. Top, CellROX Green. Bottom, phase-contrast images. Scale bar: 200µM. Typical experiment is shown and was repeated three times with similar results.
Microscopes Featuring Environmental Chambers, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microscopes featuring environmental chambers/product/Carl Zeiss
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microscopes featuring environmental chambers - by Bioz Stars, 2026-03
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microscope incubator cage with gas micro- environmental chamber and air heater  (Okolab USA Inc)
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Okolab USA Inc microscope incubator cage with gas micro- environmental chamber and air heater
(A) Live-cell imaging using Mitotracker DeepRed FM and a 100x objective of Nikon-Eclipse <t>Ti-E</t> inverted wide-field <t>microscope</t> equipped with an environmental chamber. Bottom panels, higher magnification. Left-bottom panel, quantification of mitochondrial branch length in µM from 90-100 control and USP14 deleted cells. Scale bar: 20µM. Typical experiment is shown and was repeated three times with similar results. (B) EM imaging was done as described in Methods. Note elongated mitochondria (MT) in USP14 deleted cells. Red stars * mark the presence of electron-dense lysosomes/ autophagosomes/ vesicles that were increased in the USP14-deleted cells compared with controls. N represents nuclear compartment. The experiment was repeated with similar results. (C) Left panel, immunoblot using an OXPHOS antibody cocktail. Right panel, quantification of the densitometry ratio of CI-CV subunits (CI-NDUFB8, CII-SDHB, CIII-UQCRC2, CIV-MTCO1 and CV-ATP5A) normalized to β-actin. Values are means ± S.E.M. *p ≤ 0.05, ns= not significant. n=3. (D) Live-cell imaging using CellROX Green and a 20x objective of EVOS FL microscope to visualize reactive oxygen species (ROS). Note increases of ROS in USP14-deleted cells Control cells treated with 5µM H 2 O 2 for 90 min served as a positive control for oxidative stress. Top, CellROX Green. Bottom, phase-contrast images. Scale bar: 200µM. Typical experiment is shown and was repeated three times with similar results.
Microscope Incubator Cage With Gas Micro Environmental Chamber And Air Heater, supplied by Okolab USA Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microscope incubator cage with gas micro- environmental chamber and air heater/product/Okolab USA Inc
Average 90 stars, based on 1 article reviews
microscope incubator cage with gas micro- environmental chamber and air heater - by Bioz Stars, 2026-03
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(A) Live-cell imaging using Mitotracker DeepRed FM and a 100x objective of Nikon-Eclipse Ti-E inverted wide-field microscope equipped with an environmental chamber. Bottom panels, higher magnification. Left-bottom panel, quantification of mitochondrial branch length in µM from 90-100 control and USP14 deleted cells. Scale bar: 20µM. Typical experiment is shown and was repeated three times with similar results. (B) EM imaging was done as described in Methods. Note elongated mitochondria (MT) in USP14 deleted cells. Red stars * mark the presence of electron-dense lysosomes/ autophagosomes/ vesicles that were increased in the USP14-deleted cells compared with controls. N represents nuclear compartment. The experiment was repeated with similar results. (C) Left panel, immunoblot using an OXPHOS antibody cocktail. Right panel, quantification of the densitometry ratio of CI-CV subunits (CI-NDUFB8, CII-SDHB, CIII-UQCRC2, CIV-MTCO1 and CV-ATP5A) normalized to β-actin. Values are means ± S.E.M. *p ≤ 0.05, ns= not significant. n=3. (D) Live-cell imaging using CellROX Green and a 20x objective of EVOS FL microscope to visualize reactive oxygen species (ROS). Note increases of ROS in USP14-deleted cells Control cells treated with 5µM H 2 O 2 for 90 min served as a positive control for oxidative stress. Top, CellROX Green. Bottom, phase-contrast images. Scale bar: 200µM. Typical experiment is shown and was repeated three times with similar results.

Journal: bioRxiv

Article Title: USP14 regulates pS129 α-synuclein levels and oxidative stress in human SH-SY5Y dopaminergic cells

doi: 10.1101/2024.05.09.592905

Figure Lengend Snippet: (A) Live-cell imaging using Mitotracker DeepRed FM and a 100x objective of Nikon-Eclipse Ti-E inverted wide-field microscope equipped with an environmental chamber. Bottom panels, higher magnification. Left-bottom panel, quantification of mitochondrial branch length in µM from 90-100 control and USP14 deleted cells. Scale bar: 20µM. Typical experiment is shown and was repeated three times with similar results. (B) EM imaging was done as described in Methods. Note elongated mitochondria (MT) in USP14 deleted cells. Red stars * mark the presence of electron-dense lysosomes/ autophagosomes/ vesicles that were increased in the USP14-deleted cells compared with controls. N represents nuclear compartment. The experiment was repeated with similar results. (C) Left panel, immunoblot using an OXPHOS antibody cocktail. Right panel, quantification of the densitometry ratio of CI-CV subunits (CI-NDUFB8, CII-SDHB, CIII-UQCRC2, CIV-MTCO1 and CV-ATP5A) normalized to β-actin. Values are means ± S.E.M. *p ≤ 0.05, ns= not significant. n=3. (D) Live-cell imaging using CellROX Green and a 20x objective of EVOS FL microscope to visualize reactive oxygen species (ROS). Note increases of ROS in USP14-deleted cells Control cells treated with 5µM H 2 O 2 for 90 min served as a positive control for oxidative stress. Top, CellROX Green. Bottom, phase-contrast images. Scale bar: 200µM. Typical experiment is shown and was repeated three times with similar results.

Article Snippet: The stained live-cells were imaged utilizing Nikon Eclipse Ti-E inverted widefield microscope with full environmental chamber, Hamamatsu Orca Flash 4.0 V2 B&W camera for fluorescence and Lumencor Spectra X light engine (Biomedicum Imaging Unit, Medicum, University of Helsinki).

Techniques: Live Cell Imaging, Microscopy, Imaging, Western Blot, Positive Control